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. 2018 Jul 11;23:33. doi: 10.1186/s11658-018-0099-8

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — LAMP3 regulated cell viability in osteosarcoma (OS) cells. a, b, d and e – Western blots showing the efficiency of LAMP3 knockdown in OS-732 (a, b) or U2OS (d, e) cells at the mRNA (a, d) and protein (b, e) levels. β-actin served as the loading control. g and h – Western blots showing the efficiency of LAMP3 overexpression. c, f and i – MTT assay results showing that OS cell viability decreased after the inhibition of LAMP3 (C) and increased following overexpression of LAMP3 (f) compared with the control (i). *p < 0.05, **p < 0.01, ***p < 0.001, n = 3