Table 1.
CpGs in vitamin D-related genes with statistically significant (p < 0.05) associations with 25(OH)D; Sister Study subcohort (n = 1270)
| Rank | CpG | Gene / location type | Chromosome: position | Mean methylation level (SD) | Association with 25(OH)Da | |
|---|---|---|---|---|---|---|
| β | p value | |||||
| 1 | cg21201924 | RXRA / body | 9: 137251825 | 0.76 (0.042) | −0.020 | 0.0004 |
| 2 | cg02127980 | RXRA / body | 9: 137252116 | 0.40 (0.067) | −0.015 | 0.0004 |
| 3 | cg17559402b | NADSYN1 / body | 11: 71187890 | 0.97 (0.006) | −0.017 | 0.003 |
| 4 | cg02059519 | RXRA / body | 9: 137250935 | 0.83 (0.026) | −0.012 | 0.003 |
| 5 | cg09997530 | GC / body | 4: 72636217 | 0.91 (0.017) | 0.015 | 0.005 |
| 6 | cg04329455b | RXRA | 9: 137215364 | 0.96 (0.008) | 0.014 | 0.007 |
| 7 | cg00268518b | NADSYN1 / TSS200 | 11: 71164106 | 0.01 (0.001) | 0.011 | 0.008 |
| 8 | cg03146219 | NADSYN1 / body | 11: 71189514 | 0.47 (0.097) | −0.012 | 0.009 |
| 9 | cg13510651b | RXRA / body | 9: 137227772 | 0.94 (0.008) | −0.010 | 0.01 |
| 10 | cg03490288b | DHCR7 / body | 11: 71146658 | 0.97 (0.006) | 0.015 | 0.01 |
| 11 | cg05785753 | NADSYN1 / body | 11: 71189490 | 0.59 (0.074) | −0.010 | 0.01 |
| 12 | cg13687497 | RXRA / body | 9: 137249839 | 0.80 (0.023) | −0.010 | 0.01 |
| 13 | cg07793224b | NADSYN1 / body | 11: 71183180 | 0.97 (0.008) | 0.017 | 0.02 |
| 14 | cg26044621b | DHCR7 / 3´ UTR | 11: 71145665 | 0.94 (0.011) | 0.012 | 002 |
| 15 | cg04837494 | GC / 3´ UTR | 4: 72608149 | 0.85 (0.033) | 0.013 | 0.03 |
| 16 | cg14236758 | RXRA / body | 9: 137252129 | 0.48 (0.066) | −0.009 | 0.03 |
| 17 | cg04774822 | NADSYN1 / body | 11: 71165839 | 0.78 (0.033) | −0.009 | 0.03 |
| 18 | cg16151558b | DHCR7 / TSS1500 | 11: 71159853 | 0.02 (0.066) | 0.013 | 0.04 |
| 19 | cg20372759b | CYP27B1 / TSS1500 | 12: 58162287 | 0.97 (0.006) | −0.010 | 0.04 |
| 20 | cg24806812 | GC / body | 4: 72635202 | 0.93 (0.018) | 0.012 | 0.04 |
| 21 | cg14154547b | RXRA / body | 9: 137293309 | 0.92 (0.010) | −0.007 | 0.04 |
| 22 | cg07099121b | DHCR7 / 3´ UTR | 11: 71146096 | 0.98 (0.004) | −0.010 | 004 |
| 23 | cg16910670b | NADSYN1 | 11: 71215361 | 0.97 (0.005) | −0.011 | 0.05 |
TSS200 within 200 basepairs upstream of the transcription start site, TSS1500 within 1500 basepairs upstream of the transcription start site, UTR untranslated region
aEstimated change in methylation (logit(β)) per 10 ng/mL change in serum 25-hydroxyvitamin D (25(OH)D)
bIntraclass correlation coefficient < 0.5