Table 1.
Viral sequences obtained by high throughput sequencing from sand flies pools captured in the rainy, intermediate and dry period at North Pantanal and Chapada dos Guimarães National Park, Mato Grosso, Brazil
| Place | Period | Pool | Species (n) | Reads | Contigs | Viral hits | Genome | Size (nt) | Identity (%)a | E value | GenBank ID |
|---|---|---|---|---|---|---|---|---|---|---|---|
| North Pantanal | Rainy | 1 | Evandromyia (Aldamyia) evandroi (5) | 983,899,176 | 11,747 | – | – | – | – | – | – |
| 2 | Lutzomyia sp. (25) | 849,154.470 | 61,862 | – | – | – | – | – | – | ||
| 3 | Ev. (Eva.) wilsoni (11) | 819,202,516 | 43,993 | – | – | – | – | – | – | ||
| 4 | Evandromyia (Aldamyia) carmelinoi (1) | 1,179,702,220 | 91,115 | – | – | – | – | – | – | ||
| 5 | Lu. (Tricholateralis) sherlocki (3) | 449,698,864 | 28,893 | – | – | – | – | – | – | ||
| 6 | Brumptomyia sp. (15) | 785,799,190 | 73,656 | Vesiculovirus | ssRNA- | 69 | 44.9 | 3.83e-12 | – | ||
| Transitional | 7 | Lu. longipalpis (3) | 645,865,104 | 13,763 | Phlebovirus (Seg L) | ssRNA- | 6341 | 61 | 0 | MF289183 | |
| Phlebovirus (Seg M) | ssRNA- | 4403 | 46 | 0 | MF289182 | ||||||
| Phlebovirus (Seg S) | ssRNA- | 1747 | 53 | 2e-79 | MH119632 | ||||||
| Dry | 8 | Lutzomyia sp. (5) | 1,115,860,120 | 94,199 | Vesiculovirus | ssRNA- | 72 | 40.3 | 4.10e-11 | – | |
| Flavivirus | ssRNA+ | 48 | 47.9 | 5.88e-10 | – | ||||||
| CGNP | Rainy | 9 | Lutzomyia sp. (22) | 999,786,678 | 28,460 | – | – | – | – | – | – |
| 10 | Nyssomyia whitmani (3) | 787,599,818 | 37,990 | – | – | – | – | – | – | ||
| 11 | Brumptomyia sp. (1) | – | – | – | – | – | – | – | – | ||
| Dry | 12 | Nyssomyia whitmani (12) | 692,307,934 | 30,666 | – | – | – | – | – | – |
aIdentity refers to the closest phlebovirus species Chagres virus indicated by BLASTx (GenBank)
Abbreviations: CGNP Chapada dos Guimarães National Park, n number, nt nucleotides, Seg segment