Table 2.
Summary of partial purification of crude protease from E. indicum isolate.
| Steps | Volume (ml) | Protein (mg/ml) | Total protein (mg) | Activity (U/ml) | Total activity (U) | Specific activity (U/mg) | Purification fold | Yield (%) |
|---|---|---|---|---|---|---|---|---|
| Crude | 150 | 0.457 | 68.55 | 130 | 19500 | 284.46 | 1 | 100 |
| 30% (NH4)2SO4 ppt. | 2 | 1.115 | 2.230 | 520.5 | 1041 | 466.81 | 1.46 | 5.33 |
| 60% (NH4)2SO4 ppt. | 2 | 1.884 | 3.768 | 3172.5 | 6345 | 1683.92 | 5.91 | 32.53 |
| 90% (NH4)2SO4 ppt. | 2 | 1.513 | 3.026 | 882.5 | 1765 | 583.27 | 2.05 | 9.05 |
| DEAE- Cellulose | 1 | 0.185 | 0.185 | 1835 | 1835 | 9918.91 | 34.86 | 9.41 |