Table 4.

Effects of protease inhibitors on the activity of the partially purified protease from B. subtilis and E. indicum.

Inhibitors	Concentrations	Relative residual activity (%) of purified protease from
		B. subtilis	E. indicum
Controla	None	100	100
PMSF	1 mM	23	47
1, 10 Phenanthroline	1 mM	95.5	91.4
HgCl2	1 mM	30	9.6
EDTA	1 mM	94.4	84.5
Leupeptin	25 μM	86	92.5
Pepstatin	25 μM	90.5	88.3

^aThe activity of protease treated without any protease inhibitor (control) was considered as 100%.