Fig. 1.

Long-term HighGlu does not affect differentiation, viability or proliferation of BMDMs. Bone marrow cells were cultured for 7 days under NormGlu, HighGlu or Mannitol (osmolarity control) conditions. a Flow cytometric analysis showing the percentage of F4/80⁺ cells differentiated under the three culture conditions. b MTT assay demonstrating the cell viability of BMDMs. c Graph showing the percentage of Ki67⁺ cells. d Representative images of Ki67⁺ cells (red), counterstained with DAPI (blue). e Western blot for Casp3 using protein extracts from the three culture conditions. BMDMs treated with TNFα were used as a positive control. f Representative images of TUNEL assay (red). DNase-treated BMDMs were used as a positive control. Scale bars = 25 μm. Data are represented as mean ± SEM. One-way ANOVA followed by Tukey’s multiple comparison post hoc test was performed