Fig. 6.

STK25 mediates glycolysis through GOLPH3-regulated mTOR signaling. Western blot was performed to assess the levels and phosphorylation of mTOR substrates in LoVo cells transfected with STK25 expression plasmid (a) or STK25 siRNA (b). c Knockdown of GOLPH3 partially reverses the upregulation of pAKT and pS6K induced by STK25 depletion. The relative phosphorylation levels of specific substrates of mTORC1 and mTORC2 were quantified by assessing the relative intensity of phosphorylated bands to corresponding protein bands (a-c; shown as mean ± SD of three scans). Rapamycin attenuates STK25 knockdown-induced glucose uptake (d) and lactate production (e). Data are expressed as mean ± SD. *, P < 0.05