Fig. 3.

Acute gouty inflammation was induced in the peritoneal cavity and air pouch models from miR-155 knock-out (KO) and wild-type (WT) mice treated with MSU. a, c Total cell number in lavage fluids from the peritoneal cavity (a) and air pouch (c) models were counted by a hematocytometer. d, e The mRNA expression of tumor necrosis factor (TNF)-α (d) and interleukin (IL)-1β (e) were measured by real-time qPCR in the total cells from air pouch lavage fluids of miR-155 KO and WT mice treated with MSU crystals for 0, 4, or 8 h. b, f The cytokine IL-1β levels in lavage fluids from peritoneal cavity (b) and air pouch (f) models were measured by ELISA. Results are representative of three independent experiments; n = 3–5 mice per group