Table 1.

Primary and secondary outcomes and moment of evaluation accordingly to study design.

Type of Variables	Baseline	Before each meal	After each meal	After exercise challenge	Wash out-period	After induced physical activity reduction
Primary outcome and immune response outcomes
		IL-6				IL-6
		Peripheral blood lymphocyte subpopulations, including NK, B, T and T reg relative numbers		Peripheral blood lymphocyte subpopulations, including NK, B, T and T reg relative numbers		Peripheral blood lymphocyte subpopulations, including NK, B, T and T reg relative numbers
		TNF-α; IL8; IL2; IL10; TGF β; IL1RA				TNF-α; IL8; IL2; IL10; TGF β; IL1RA
Secondary outcomes
	Anthropometry: weight, body mass index, percentage of body fat and fat free mass					Anthropometry: weight, body mass index, percentage of body fat and fat free mass
	Lung function, airway inflammation					Lung function, airway inflammation
		Adipokine				Adipokine
		Allostatic load				Allostatic load
		Pupillometry and substance P				Pupillometry and substance P
		Metabolomic markers, pH, Na, K+ and conductivity in EBC				Metabolomic markers, pH, Na, K+ and conductivity in EBC
		Primary and oxidative DNA damage		Primary and oxidative DNA damage
Potential confounding factors	Age, gender, smoking habits, physical activity, dietary intake, atopy, asthma diagnosis and control, education and occupation, pupillometry, Anxiety and depression questionnaire	Asthma control questionnaire, Anthropometry: weight, height, BMI, waist circumference, percentage of body fat and fat free mass	Meal taste score	Physical fitness: cardiorespiratory fitness	Accelerometer & Pedometer 3 day Food consumption diary	Anxiety, depression and insomnia questionnaires