FIGURE 4.
Expression of CcMYB12 transgene (A) and of endogenous phenylpropanoid biosynthetic genes (B) in leaves of transgenic Arabidopsis plants. qRT-PCR experiments were conducted on cDNAs from various transgenic Arabidopsis T3 plants grouped in 4 different pools (pool 1, 2, 3, and 4), each constituted by 4 plants of the same transgenic T3 line. Actin was employed as reference gene. (A) qRT-PCR of CcMYB12 in transgenic Arabidopsis plants. Pool 4 as calibrator. (B) Expression levels of PAL, CHI, FLS, CHS, F3H, F3′H, DFR, ANS, AtMYB12 and AtMYB111 in CcMYB12 transgenic Arabidopsis plants. WT as calibrator. Values are means ± SD of three biological replicates. Asterisks indicate a statistical difference (∗p-value < 0.05; ∗∗p-value < 0.01) between the means for WT and tested transgenic samples, according to Student’s t-test.