Figure 2. Influence of long-term storage at −80 °C and physical and mechanical disturbances.

(A) Clear serum samples were stored at −80 °C for 7 months prior to testing anti-PLA2R antibodies. (B) Clear serum samples were stored at −80 °C for 12 months prior to testing anti-PLA2R antibodies. (C) Clear serum samples were placed on a shaker at 110 rpm at room temperature for 1 or 8 h of shaking prior to testing anti-PLA2R antibodies. *p < 0.05.