Table 1. High frequency mutations observed.

Bases	Genome change	Protein change	Frequency
1,084–3,212	Deletion	Loss of 0.4–0.7	1.0*
32,855	T→C	I754T (16)	0.89
33,491	C→T	A966V (16)	1.0
35,095	A→G	N158D (17)	1.0*

Notes:

This table includes all changes above a frequency of 15% in cycles 11 or 30. Those listed are from cycle 30; mutations seen in cycle 11 are a subset of those seen at 30 and are indicated with a superscript ^* in the last column (both were at frequency of 1.0 in cycle 11). Two mutations differing from the published wild-type sequence were present in the ancestor used to start the adaptation: 15094 G→T (A248S, gp5) and 29258 A→G (K312E, gp15).