Table 4.

RUNX3

Author/year	Patient no (P:NP)	Methods	Methylation threshold definition	Statistical analysis	Result
Boerwinkel et al 201443	44 (48:10)	Methylight methylation-specific PCR	Previously published cut-off values applied to raw MSP data to calculate frequency of hypermethylation. RUNX3 cut-off=0.02	Mann-Whitney test	Raw MSP values compared but no numerical value offered P versus NP not significantly differentially methylated
Jin et al 200941	195* (50:145)	Methylation-specific PCR	NMV=amount of methylated DNA compared with control beta-actin DNA generated by PCR	Student’s t test and chi-squared test AUROC	Hypermethylated in P NMV 0.104:0.063 AUROC=0.671 (0.586, 0.756) 90% sensitivity 90% specificity P=0.0002
Sato et al 200840	62 (28:34)	Methylation-specific PCR	NMV=amount of methylated DNA compared with control beta-actin DNA generated by PCR	LDA, LOOCV, AUROC	Hypermethylated in P AUROC increment when added to segment length, histology and global methylation index=0.0216 90% sensitivity 90% specificity P=0.016
Schulmann et al 200539	53 (8:45)	Methylation-specific PCR	NMV=amount of methylated DNA compared with control beta-actin DNA generated by PCR	Cox proportional HRs	Hypermethylated in P OR=1.80 P=0.0267

*Number of lesions, no patient numbers described in the study; number of progressor lesions (P), number of non-progressor lesions (NP).

AUROC, area under receiver operating characteristic; LDA, linear discriminant analysis; LOOCV, leave-one-out cross-validation; MSP, methylation specific PCR; NMV, normalised methylation value; NP, non-progressing patients; P, progressing patients.