Table 5.

HPP1

Author/year	Patient no (P:NP)	Methods	Methylation threshold definition	Statistical analysis	Result
Boerwinkel et al 201443	44 (48:10)	Methylight methylation-specific PCR	Previously published cut-off values applied to raw MSP data to calculate frequency of hypermethylation. HPP1 cut-off=0.05	Mann-Whitney test	P versus NP not significantly differentially methylated
Jin et al 200941	195* (50:145)	Methylation-specific PCR	NMV=amount of methylated DNA compared with control beta-actin DNA generated by PCR	Student’s t test and chi-squared test AUROC	Hypermethylated in P AUROC=0.647 (0.556, 0.739) P=0.0025
Sato et al 200840	62 (28:34)	Methylation-specific PCR	NMV=amount of methylated DNA compared with control beta-actin DNA generated by PCR	LDA, LOOCV, AUROC	Hypermethylated in P AUROC increment when added to segment length, histology and global methylation index 0.028 90% sensitivity 90% specificity P=0.018
Schulmann et al 200539	53 (8:45)	Methylation-specific PCR	NMV=amount of methylated DNA compared with control beta-actin DNA generated by PCR	Cox proportional HRs	Hypermethylated in P OR=1.77 P=0.0311

*Number of lesions, no patient numbers described in the study; number of progressor lesions (P), number of non-progressor lesions (NP).

AUROC, area under receiver operating characteristic; LDA, linear discriminant analysis; LOOCV, leave-one-out cross-validation; MSP, Methylation Specific PCR; NP, non-progressing patients; P, progressing patients.