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. Author manuscript; available in PMC: 2018 Jul 12.
Published in final edited form as: Cell Transplant. 2015 Mar 6;25(1):35–53. doi: 10.3727/096368915X687697

Figure 3.

Figure 3

Effects of hypoxic preconditioning on CDC mRNA and protein expression levels. (A) The relative mRNA or protein expression of HIF-1α, VEGF, EPO, CXCR-4, TERT, CD105, c-Kit, CD90, Oct-4, Klf-4, Sox 2, Nanog, Nkx 2.5, GATA 4, Troponin T (Tnnt), and myosin heavy chain (MyHC) for normoxic P2 CDCs (represented by black boxes) and hypoxic P2 CDCs (represented by white boxes) (n = 3). (B) Representative confocal images (of three independent experiments) of the immunostaining for c-Kit, CD90, Oct-4, Klf-4, Sox 2, and Nanog for normoxic P2 CDCs and hypoxic P2 CDCs. *p < 0.01 versus normoxic P2 CDCs. Green fluorescence indicates the specific antibody while blue fluorescence indicates DAPI staining. The white boxes indicated the zoomed in area. Scale bars: 50 µm.

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