Figure 8.
Metabolic adaptation of normoxic CDCs, hypoxic CDCs, and CDCs treated with 1 mM DMOG and 30 µM BIC for 24 h. (A) Basal and FCCP-uncoupled respiration rates were reduced despite unchanged mitochondrial numbers indicated by (B) unchanged citrate synthase protein levels. (C) GLUT-1 protein expression, (D) levels of glucose uptake and production of lactate were increased, suggesting that hypoxic and PHDI-preconditioned cells switched to more glycolytic metabolism (n = 3). *p < 0.05 vs. control. All experiments in this study used P2 CDCs at 70% to 80% confluency, unless otherwise stated.