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a) Raman spectra at increasing depths into tissue. TFV contributions are visible at the 725 cm−1 peak (boxed) and were seen to drop to negligible levels beyond 360 μm. Spectra beyond 360 μm corresponded to Raman shifts characteristic of lipid[33]. b) The change in spectral trace correlated to a change in tissue morphology observable with OCT. A=adipose tissue, M=mucosal tissue (epithelium and stroma).
