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. Author manuscript; available in PMC: 2018 Jul 12.
Published in final edited form as: J Struct Biol. 2017 Jun 20;200(3):293–302. doi: 10.1016/j.jsb.2017.06.007

Fig. 7.

Fig. 7.

Effects of cholesterol on the overall structure and stability of SAA:POPC complexes. The complexes were prepared by cholate dialysis method using SAA:POPC: Cholesterol molar ratios of 1:30:2 or 1:80:4 as indicated. Cholesterol-free complexes containing 1:30 or 1:80mol:mol SAA:POPC are shown for comparison. These experiments were repeated twice using two independent sets of lipoprotein preparations. (A) Native PAGE of intact complexes (E-Z Run protein stain); lipid-free SAA is shown for comparison. (B) Far-UV CD spectra of intact complexes at 25 °C. (C) CD heating and cooling data of the complexes. Line coding is as in panel B. Arrows show directions of temperature changes. (D) Native PAGE of SAA:POPC and SAA:POPC:cholesterol complexes that have been either unheated (25 °C) or have been heated up to 60 °C. Asterisks indicate HDL-size particles formed upon thermal denaturation of intact lipoproteins.