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. 2018 Jul 12;13(7):e0200487. doi: 10.1371/journal.pone.0200487

Fig 6. AIF1L interacts with components of the actomyosin machinery and nuclear proteins.

Fig 6

(a) Schematic depicting the labeling strategy of wild type and AIF1L knockout clones employing the SILAC technology, and the consecutive immunoprecipitation as well as sample preparation for analysis via mass spectrometry. (b) Color-coded tabular presentation of proteins according to their enrichment score (normalized to the AIF1L knockout control) in two replicates. Red colored protein names belong to the non-muscle myosin-II actomyosin machinery. (c) GO-Term mapping of interaction partners from the knockout controlled immunoprecipitation experiments. Aside from proteins belonging to the actomyosin machinery, also ERM proteins and chromatin regulating proteins were mainly detected. (d) Validation experiments employing western blot confirm the association of MYL9, MYH9 and the chaperone UNC45A with AIF1L.