Skip to main content
. 2018 Jun 29;14(6):e1007135. doi: 10.1371/journal.ppat.1007135

Fig 1. Effect of the FMDV leader protease on LGP2.

Fig 1

(A) HEK293 cells were mock-transfected (-) or co-transfected with a plasmid encoding hLGP2-Myc-DDK and empty vector (EV), LbWT or LbC51A. Cells were lysed 24 h later and analyzed by western blot for the indicated proteins with the specified antibodies. The N- and C-terminal cleavage products of LGP2 are indicated with arrows. (B) HEK293 cells were mock-transfected (-), transfected with EV, or with plasmids encoding LbWT or LbC51A and lysed 24 h later (left and middle panels); BHK-21 cells were mock-infected (-) or infected with FMDV O1BFS at an MOI of 5 and lysed 5 h later (right panel). Lysates were analyzed by western blot for detection of eIF4G cleavage products (arrowhead). (C) HEK293 cells were mock-transfected (-) or co-transfected with 500ng of a plasmid encoding DDK-METTL3 and 500ng of empty vector (EV), LbWT or LbC51A.