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. Author manuscript; available in PMC: 2018 Jul 12.
Published in final edited form as: J Pathol. 2017 Jul 27;243(1):65–77. doi: 10.1002/path.4928

Figure 4. GSK-3β is necessary for KRas-initiated ADM in vivo.

Figure 4.

(A) Scheme for caerulein-induced acute pancreatitis model and analysis. (B) Gross pathology of pancreas and adjacent tissues from transgenic mice of indicated genotypes treated with saline or 2 and 7 days post caerulein injection. (C) H&E stained pancreatic sections from WT, KRas or RKO mice (left panel) and immunofluorescence staining of amylase and CK19 (right panel) of mice treated with saline or 2 and 7 days post caerulein injection. Nuclei were counter-stained with Hoechst (blue). (D) H&E stained tissue samples from WT (black), KRas (red), KO (grey) and RKO (green) mice, respectively, were evaluated and quantitatively analyzed for ADM area as percentage to the total area. Data were analyzed and expressed as mean ± SEM. n = 25. *P<0.05 KRas versus WT mice. #P<0.05 RKO versus KRas mice. (E) Real-time PCR quantification of pancreatic gene expression for amylase (black), CK7 (red) and CK19 (blue) from WT, KRas or RKO mice treated with saline or 2, 7 days post caerulein injection. RPLP0 and GAPDH were used as internal housekeeping gene controls. Data were analyzed and expressed as mean ± SEM. n = 5. *P<0.05 KRas versus WT mice. #P<0.05 RKO versus KRas mice.