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. Author manuscript; available in PMC: 2018 Jul 12.
Published in final edited form as: J Pathol. 2017 Jul 27;243(1):65–77. doi: 10.1002/path.4928

Figure 6. GSK-3β deletion decreases the levels of phospho-S6.

Figure 6.

(A) Double-labeling of the pancreatic sections for pS6 (red) and CK19 (green). The pancreatic sections were derived from KRas and RKO mice 2 or 7 days post caerulein injection. Nuclei were counter-stained with Hoechst (blue). (B) AR42J cells were pre-treated with diluent (DMSO) or GSK-3i for 2 hours. They were then treated with caerulein for an additional 2 or 6 hours. Cell lysates were prepared and probed with the indicated antibodies. (C) Stable control and shGSK-3β AR42J cells were treated with caerulein for 2, 6 and 12 hours. Cell lysates were prepared and probed with the indicated antibodies. Shown are representative results from 6 independent experiments.