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. Author manuscript; available in PMC: 2018 Jul 12.
Published in final edited form as: J Alcohol Drug Depend. 2018 Apr 30;6(2):312. doi: 10.4172/2329-6488.1000312

Figure 2.

Figure 2

Double immunofluorescence labeling demonstrating the location of GAD67-GFP positive cells with respect to the tyrosine hydroxylase (TH) immunoreactive (IR) dopaminergic neurons of ventral tegmental area (VTA). Panels A–C: High power images showing GAD67-GFP positive cells (A), TH IR cells (B) and merged images of GAD67-GFP and TH in areas overlapping VTA, SNC and SNCM. Panels D–F: High power images showing GAD67-GFP positive cells (D), TH IR cells (E) and merged images of GAD67-GFP areas overlapping VTA and RPC. GABAergic neurons are seen in close proximity to the dopaminergic neurons of VTA.

Solid arrows point to representative GAD67-GFP positive GABAergic cells and broken arrows to TH IR dopaminergic cells. Abbreviations: VTAR= ventral tegmental area rostral, SNCM= substantia nigra compact part, medial tier, fr= fasciculus retroflexus, RPC= red nucleus parvocellular part.