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. 2018 Jun 13;7:e35012. doi: 10.7554/eLife.35012

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© 2018, Künzel et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 5. — (A, B) Immunofluorescence of iRhom1/2 double knockout HEK293T cells stably expressing iRhom2-3xHA or iRhom2^Δ300-3xHA and transiently transfected with FRMD8-V5 for 72 hr. Cells were stained for HA (red), V5 (green) and DAPI for DNA (blue). Single confocal sections are shown, taken through the centre of the nucleus. (C) Schematic model of the FRMD8-iRhom2^Δ300 construct used in (E). (D, E) Immunofluorescence of iRhom1/2 double knockout HEK293T cells stably expressing iRhom2^Δ300-3xHA or FRMD8-iRhom2^Δ300-3xHA and transiently transfected with ADAM17-V5 for 72 hr. Cells were stained for HA (green), V5 (red) and DAPI for DNA (blue). Single confocal sections are shown, taken either through the centre of the nucleus (MEDIAL), or at basal regions close to the coverslip (BASAL). In all images the scale bar = 10 µm.