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. Author manuscript; available in PMC: 2018 Oct 17.
Published in final edited form as: Oncogene. 2018 Apr 17;37(28):3879–3893. doi: 10.1038/s41388-018-0239-7

Figure 1. Overexpression of miR-106b-25 upregulates Notch1 signaling.

Figure 1

(A) Western blot analysis for NOTCH1-ICD and Full length (FL) NOTCH1 in a panel of breast cancer cell lines with stable overexpression of the miR-106b-25 cluster (CLR) including miR-106b, miR-93, and miR-25 or a non-silencing control (NS). (B) Notch Luciferase reporter assay using the RBP-Jk reporter transfected into MCF7, T47D, HCC1937, Sum159, and Sum149 NS and Cluster expressing lines. Statistical significance assessed by a student T-test on triplicate samples of a representative experiment (n=3). (C) qRT-PCR analysis in MCF7 and Sum159 NS and CLR cells showing an increase in NOTCH1 direct target genes with CLR overexpression. Statistical significance assessed by a student T-test on six replicate samples of a representative experiment (n=2).