(A) Analysis of animal survival in mice with or without repeated intraperitoneal
administration of vitamin E (500 mg/kg) at three, 24, 48, and 72 hours after CLP
(22-gauge needle)-induced sepsis (n=5–10 mice/group; *,
P<0.05, Kaplan-Meier survival analysis). (B) In
parallel, serum levels of MDA, 4-HNE, CK, BUN, and ALT were assayed at 72 hours
after CLP (n=5 mice/group; *, P<0.05, ANOVA
LSD test). Data are presented as median value (black line),
interquartile range (box), and minimum and maximum of all data (black line). (C)
Analysis of animal survival in mice with or without repeated intraperitoneal
administration of Z-VAD-FMK (“ZVF”, 5 mg/kg), wedelolactone
(“Wed”, 20 mg/kg), ferrostatin-1 (“Fer”, 10
mg/kg), or necrostatin-1 (“Nec”, 5 mg/kg) at three, 24, 48, and
72 hours after CLP (22-gauge needle)-induced sepsis (n=10 mice/group;
*, P<0.05, Kaplan-Meier survival analysis). (D) In
parallel, serum levels of IL-1β, IL-18, HMGB1, IL-6, IL-10, IL-17, and
IL-12 were assayed at 72 hours after CLP (n=5 mice/group; *,
P<0.05, ANOVA LSD test). Data are
presented as median value (black line), interquartile range (box), and minimum
and maximum of all data (black line).