REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal [EPNCIR144] to GPX4	Abcam	ab125066, RRID:AB_10973901
Rabbit polyclonal to caspase-1	Abcam	ab1872, RRID:AB_302644
Goat polyclonal to IL-1β	R&D Systems	AF-401-NA, RRID:AB_416684
Rabbit polyclonal to GSDMD	Sigma Aldrich	G7422, RRID:AB_1850381
Mouse monoclonal to caspase-1 (p20)	AdipoGen	AG-20B-0042, RRID:AB_2490248
Mouse monoclonal [A-7] to GSDMD	Santa Cruz Biotechnology	Sc-393656, RRID: AB_2728694
Mouse monoclonal [64-Y] to GSDMD	Santa Cruz Biotechnology	Sc-81868, RRID: RRID:AB_2263768
Rabbit polyclonal to PLCG1	Cell Signaling Technology	2822, RRID:AB_10691689
Rabbit monoclonal [D6M9S] to phospho-PLCG1 (Tyr783)	Cell Signaling Technology	14008, RRID: AB_2728690
Rabbit monoclonal [D9D6S] to PLCB3	Cell Signaling Technology	14247, RRID: AB_2728691
Rabbit monoclonal [D8K2R] to phospho-PLCB3 (Ser537)	Cell Signaling Technology	29021, RRID: AB_2728692
Mouse monoclonal [8H10D10] to actin	Cell Signaling Technology	3700, RRID:AB_2242334
Rat monoclonal [17D9] to caspase-11 (p20)	Cell Signaling Technology	14340, RRID: AB_2728693
Rabbit polyclonal to HMGB1	Cell Signaling Technology	2822, RRID:AB_10691689
Chemicals, Peptides, and Recombinant Proteins
Escherichia coli LPS 0111:B4	Sigma Aldrich	L4391
Vitamin E	Sigma Aldrich	258024
Necrostatin-1	Sigma Aldrich	N9037
Z-VAD-FMK	Sigma Aldrich	V116
Wedelolactone	Sigma Aldrich	W4016
PA	Sigma Aldrich	P9511
DAG	Sigma Aldrich	D8394
Linoleic acid	Sigma Aldrich	L1376
Ceramide	Sigma Aldrich	22244
Cholesterol	Sigma Aldrich	C8667
U73122	Sigma Aldrich	662035
Gentamicin	Sigma Aldrich	G1397
Ferrostatin-1	Selleck Chemicals	S7243
ATP	InvivoGen	tlrl-atp
Poly(I:C)	InvivoGen	31852-29-6
Pam3CSK4	InvivoGen	tlrl-pms
PI4P	Echelon Biosciences Incorporated	P-4004
PI(4,5)P2	Echelon Biosciences Incorporated	P-4508
BAPTA-AM	Calbiochem	126150-97-8
Lipofectamine 3000	Invitrogen	L3000-015
Puromycin	InvivoGen	ant-pr-1
SuperSignal™ West Pico Chemiluminescent Substrate	Thermo Fisher Scientific	34080
SuperSignal™ West Femto Maximum Sensitivity Substrate	Thermo Fisher Scientific	34095
Critical Commercial Assays
Cell Counting Kit-8 (CCK-8)	Dojindo Laboratories	CK04
HMGB1 ELISA Kit	Shino Test Corporation	ST51011
IL-1β ELISA Kit	R&D Systems	MLB00C
IL-18 ELISA Kit	R&D Systems	7625
IL-12 ELISA Kit	R&D Systems	DY419
IL-17 ELISA Kit	R&D Systems	DY421
LDH Assay Kit	Abcam	ab102526
MDA Assay Kit	Abcam	ab118970
4-HNE Assay Kit	MyBioSource	MBS702101
DAG Assay Kit	Cell Biolabs Inc.	MET-5028
IP3 Assay Kit	LifeSpan Biosciences, Inc.	LS-F10644-1
CK single-slide test	IDEXX	98-11073-01
BUN single-slide test	IDEXX	98-11070-01
ALT single-slide test	IDEXX	98-11067-01
iScript cDNA Synthesis Kit	Bio-Rad	1708890
4–12% Criterion XT Bis-Tris gels	Bio-Rad	3450124
PVDF membranes	Bio-Rad	1620233
Experimental Models: Cell Lines
THP1	ATCC	TIB-202
HL-60	ATCC	CCL-240
HeLa	ATCC	CCL-2
Escherichia coli	ATCC	11775
Experimental Models: Organisms/Strains
GsdmdI105N/I105N mice	(Kayagaki et al., 2015)	N/A
Casp11 flox/flox mice	This study	N/A
Gpx4 flox/flox mice	(Yoo et al., 2012)	N/A
LysM-Cre mice	The Jackson Laboratory	004781
Alb-Cre mice	The Jackson Laboratory	003574
Nalp3−/− mice	The Jackson Laboratory	021302
Casp1−/−Casp11−/− mice	The Jackson Laboratory	016621
Oligonucleotides
Mouse Plcg1-shRNA-1 (Sequence:CCGGGCCAGCTTGTAGCACTCAATTCTCGAGAATTGAGTGCTACAAGCTGGCTTTTT)	Sigma Aldrich	TRCN0000024974
Mouse Plcg1-shRNA-2 (Sequence:CCGGCCAACTTTCAAGTGTGCAGTACTCGAGTACTGCACACTTGAAAGTTGGTTTTT)	Sigma Aldrich	TRCN0000024976
Human Plcg1-shRNA (Sequence:GTACCGGAGAAGTTCCTTCAGTACAATCCTCGAGGATTGTACTGAAGGAACTTCTTTTTTTG)	Sigma Aldrich	TRCN0000218478
See Table S2 for primers used for qPCR	This paper	Table S2
Software and Algorithms
Image Lab™ software	Bio-Rad	1709691
GraphPad Prism 7	GraphPad Software	https://www.graphpad.com/scientific-software/prism/