Table 1.
Characteristics of Parental and Reassortant Viruses in Cell Culture and in Mice
| Virus name | Reverse genetics background | TCID50 in MDCKa | 0.1 LD50 in miceb |
|---|---|---|---|
| A/swine/TX/4199-2/98-H3N2 (TX98) | TX98 | 7.625 | 5.625 |
| A/Puerto Rico/34-H1N1 (PR8) | PR8 | 9.17 | 1.55 |
| TX98:PR8 (7:1)c PR8 PB2 | TX98 | 6.50 | 5.625 |
| TX98:PR8 (7:1) PR8 PB1 | TX98 | 6.38 | 5.625 |
| TX98:PR8 (7:1) PR8 PA | TX98 | 8.00 | 5.625 |
| TX98:PR8 (7:1) PR8 HA | TX98 | 7.00 | 1.0 |
| TX98:PR8 (7:1) PR8 NP | TX98 | 7.00 | 5.625 |
| TX98:PR8 (7:1) PR8 NA | TX98 | 7.63 | 5.625 |
| TX98:PR8 (7:1) PR8 M | TX98 | 8.5 | 5.625 |
| TX98:PR8 (7:1) PR8 NS | TX98 | 7.125 | 5.625 |
| PR8:TX98 (7:1) TX98 HA | PR8 | 7.63 | 5.0 |
a
Values are reported as log10 TCID50 per milliliter.
b
Values are reported as the log10 TCID50 that represented 0.1 LD50 in BALB/c mice.
c
Virus created with seven gene segments from TX98 and one gene segment from PR8.
LD50, 50% lethal dose; MDCK, Madin-Darby canine kidney; TCID50, 50% infectious dose in tissue culture.