Table 1.
Gene name | Primer name | Primer sequence (5′-3′) | Size (bp) | Tm (°C) |
---|---|---|---|---|
AdipoQ | ADF | CACCTGAGGGACTCAGGAT | 1,163 | 59.5 |
ADR | ACTTAGGCACACAGCTCTC | 142 | 59.5 | |
ADQF | GTTCCCATTCGCTTTACCAA | 735 | 59.0 | |
ADQR | GGCTGACCTTCACATCCTTC | |||
Hind III | CCAAGCTTATGCTGCTGCTGGGAGCTCT | |||
BamH I | CGGGATCCCATTCGATGTTATGGTAGAGAA | |||
AdpoR1 | ADR1F | TGATGTCGTCTCACAAGGGA | 1,132 | 62.0 |
ADR1R | GCTCAGAGGAGGGAGTCGT | |||
ADR1QF | GCTGGGCTTTGTGCTGTTTC | 100 | 61.0 | |
ADR1QR | CATCCCGAACACCACCTT | |||
AdpoR2 | ADR2F | CCTCTTTGTCAGAAAGCCT | 1,221 | 59.5 |
ADR2R | ACCTCCAGCCAACGTCTCA | |||
ADR2QF | GAGCGGTGACAGTGATGC | 211 | 61.0 | |
ADR2QR | ACCTTCCCAGACCTTACAGA | |||
ACC | ACC-F | CCGTCTGTGATGACTTTGA | 191 | 56.4 |
ACC-R | CTTTCTGGGTTGGGTGAG | |||
FASN | FASN-F | GAAGGGTGTTGACCTCGTCC | 126 | 58.4 |
FASN-R | AGAGGGTGGTTGTTGGAAAG | |||
SREBP-1 | SREBP1-F | CTGCTGACCGACATAGAAGACAT | 81 | 56.4 |
SREBP1-R | GTAGGGCGGGTCAAACAGG | |||
C/EBPα | CEBP-F | CCGTGGACAAGAACAGCAA | 141 | 58.4 |
CEBP-R | GGCGGTCATTGTCACTGG | |||
PPARγ | PPAR1-F | GTGTCACTCCTGAACGAAAT | 156 | 56.4 |
PPAR1-R | GGAAATGCTGGAGAAGTCAA | |||
β-actin | BA-F | CCTGCGGCATTCACGAAACTAC | 87 | 59.7 |
BA-R | ACAGCACCGTGTTGGCGTAGAG |
AdipoQ, adiponectin; AdpoR, adiponectin receptor; ACC, acetyl-CoA carboxylase; FASN, fatty-acid synthase; SREBP-1, sterol regulatory element-binding protein-1; C/EBPα, CCAAT/enhancer-binding protein-α; PPARγ, peroxisome proliferator-activated receptor γ.