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. 2018 Jan 30;31(8):1088–1097. doi: 10.5713/ajas.17.0407

Table 1.

Primer pairs used in this study

Gene name Primer name Primer sequence (5′-3′) Size (bp) Tm (°C)
AdipoQ ADF CACCTGAGGGACTCAGGAT 1,163 59.5
ADR ACTTAGGCACACAGCTCTC 142 59.5
ADQF GTTCCCATTCGCTTTACCAA 735 59.0
ADQR GGCTGACCTTCACATCCTTC
Hind III CCAAGCTTATGCTGCTGCTGGGAGCTCT
BamH I CGGGATCCCATTCGATGTTATGGTAGAGAA
AdpoR1 ADR1F TGATGTCGTCTCACAAGGGA 1,132 62.0
ADR1R GCTCAGAGGAGGGAGTCGT
ADR1QF GCTGGGCTTTGTGCTGTTTC 100 61.0
ADR1QR CATCCCGAACACCACCTT
AdpoR2 ADR2F CCTCTTTGTCAGAAAGCCT 1,221 59.5
ADR2R ACCTCCAGCCAACGTCTCA
ADR2QF GAGCGGTGACAGTGATGC 211 61.0
ADR2QR ACCTTCCCAGACCTTACAGA
ACC ACC-F CCGTCTGTGATGACTTTGA 191 56.4
ACC-R CTTTCTGGGTTGGGTGAG
FASN FASN-F GAAGGGTGTTGACCTCGTCC 126 58.4
FASN-R AGAGGGTGGTTGTTGGAAAG
SREBP-1 SREBP1-F CTGCTGACCGACATAGAAGACAT 81 56.4
SREBP1-R GTAGGGCGGGTCAAACAGG
C/EBPα CEBP-F CCGTGGACAAGAACAGCAA 141 58.4
CEBP-R GGCGGTCATTGTCACTGG
PPARγ PPAR1-F GTGTCACTCCTGAACGAAAT 156 56.4
PPAR1-R GGAAATGCTGGAGAAGTCAA
β-actin BA-F CCTGCGGCATTCACGAAACTAC 87 59.7
BA-R ACAGCACCGTGTTGGCGTAGAG

AdipoQ, adiponectin; AdpoR, adiponectin receptor; ACC, acetyl-CoA carboxylase; FASN, fatty-acid synthase; SREBP-1, sterol regulatory element-binding protein-1; C/EBPα, CCAAT/enhancer-binding protein-α; PPARγ, peroxisome proliferator-activated receptor γ.