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. 2018 Jul 12;8:10491. doi: 10.1038/s41598-018-28890-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic diagram of full-length ZIKV infectious cDNA clone (a) Artifical synthetic DNA sequences 1&2 that contain ZIKV cDNA and in vitro transcription elements (T7 promoter and Ribozyme). Full-length ZIKA cDNA was designed to construct into pFK vector by using indicated restriction enzymes (Sbf1/Afe1/Mlu1). (b) Artificial synthetic cDNA sequences 1&2 were inserted into low-copy plasmid pFK, respectively. After transformation, ten colonies (numbers as indicated) were picked for each and then purified plasmids were digested by restriction enzyme (Sbf1/Afe1 or Afe1/Mlu1), respectively.