Table 1.

Prediction and silent mutation of bacterial promoter sequences within nt 1 to 3000 of the ZIKA (KU321639.1) genome.

Virus and E. coli promoter		Virus sequence (5′-3′)		Viral protein	Score
1 WT	798	GCTTTTGGGAAGCTCAACGAGCCAAAAAGTCATATACTTGGTCATGATAC	847	prM	0.98
Mutant		GCTTTTGGGTAGCTCAACGAGCCAAAAAGTCATATACTTGGTCATCATAC			ND
2 WT	1325	TCGTTAATGACACAGGACATGAAACTGATGAGAATAGAGCGAAGGTTGA	1373	E	0.97
Mutant		TCGTTAACGATACAGGACATGAAACTGATGAGAACAGAGCGAAGGTTGA			ND
3 WT	1729	GGCCACTTGAAATGTCGCCTGAAAATGGATAAACTTAGATTGAAGGGCG	1777	E	0.96
Mutant		GGCCACTTAAAGTGTCGCCTGAAAATGGACAAACTTAGATTGAAGGGCG			ND
4 WT	2098	GCATTTGAAGCCACTGTGAGAGGTGCCAAGAGAATGGCAGTCTTGGGAG	2146	E	0.94
Mutant		GCATTCGAAGCCACTGTGAGAGGTGCCAAGAGGATGGCAGTCTTGGGAG			ND
5 WT	2234	CATTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAACGTTG	2283	E	0.99
Mutant		CATTGTTCGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAACGTTG			ND
6 WT	2565	AAGAATGGAAAACATCATGTGGAGATCAGTAGAAGGGGAGCTCAACGCA	2613	NS1	0.94
Mutant		AAGAATGGAGAACATCATGTGGAGATCAGTAGAGGGGGAGCTCAACGCA			ND
7 WT	2631	AGTTCAACTGACGGTCGTTGTGGGATCTGTGAAAAACCCCATGTGGAGAG	2680	NS1	0.90
Mutant		AGTTCAACTAACGGTCGTTGTGGGATCTGTCAAAAACCCCATGTGGAGAG			ND
8 WT	2838	CTTTCTTGTGGAGGATCATGGGTTCGGGGTATTTCACACTAGTGTCTGGC	2887	NS1	0.96
Mutant		CTTTCTCGTGGAGGATCATGGGTTCGGGGTGTTTCACACTAGTGTCTGGC			ND

The underlined nucleotides correspond to the mutated ones.

ND, not detectable.