Fig. 5.

TIRR regulates DSB repair in the BRCA1-deficient cells. Wild-type TIRR or its mutants were expressed in UWB1 (BRCA1-deficient) cells. The cells were treated with 10 Gy of IR. The IRIF of RPA (a) or RAD51 (b) was examined by immunofluorescence staining. Alternatively, the cells were treated with indicated dose of olaparib (PARP inhibitor) for 6 days. Scale bar represents 5 µm. Cell viability was examined (c). Data are represented as mean ± s.d. as indicated from three independent experiments. N.S.: non significant; *: statistically significant (p < 0.05); ***: statistically significant (p < 0.001). Scale bar represents 5 µm