Table 1.
Annexin V/PI apoptosis analysis of A549 cell populations, as determined by flow-cytometric quantification (Average of triplicates ± SEM).
No apoptosis | Early apoptosis | Late apoptosis and necrosis | |
---|---|---|---|
24 h | |||
Control | 87.9 ± 3 | 1.3 ± 0.6 | 10.8 ± 2.4 |
Lipofectamine | 73.9 ± 9 | 1.8 ± 0.5 | 24.3 ± 8.5 |
Scramble | 67.9 ± 9.5 | 2 ± 0.9 | 30.1 ± 8.6 |
miR-143 | 62.3 ± 2.7 | 5.5 ± 1.8 | 32.1 ± 4.4 |
miR-506 | 55.7 ± 4.6 | 7.2 ± 2.7 | 37.2 ± 7.3 |
miR-143/506 | 50.6 ± 8.3 | 4.3 ± 0.7 | 45.2 ± 8.4 |
48 h | |||
Control | 91.8 ± 0.7 | 0.3 ± 0 | 7.9 ± 0.7 |
Lipofectamine | 84.6 ± 2.5 | 0.6 ± 0.3 | 14.8 ± 2.2 |
Scramble | 85 ± 2.4 | 0.7 ± 0.4 | 14.3 ± 2.2 |
miR-143 | 67.4 ± 4.4 | 1.2 ± 0.1 | 31.4 ± 4.3 |
miR-506 | 52.5 ± 1.4 | 1.3 ± 0.1 | 46.2 ± 1.3 |
miR-143/506 | 41.6 ± 2.8 | 1.3 ± 0.1 | 57.1 ± 2.7 |