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. 2018 Jul 12;8:10552. doi: 10.1038/s41598-018-28908-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A midpoint rooted phylogenetic tree of the B. burgdorferi chromosome generated via the SNVPhyl pipeline. All sequences obtained in this study and currently available published full sequences from samples collected elsewhere in North America and Europe were used. Colour coding indicates the geographic occurrence of the MLST types of the sequences: blue = USA only; red = Canada only, yellow = USA and Canada; violet = Europe. Red stars to the right of the tree indicate sequences obtain from Canadian sites in this study. Letters to the right of the tree indicate the ospC major groups of the strains. Letters with asterisks indicate novel ospC major group sequences as described in the text. The position of the reference strain, B31 is shown by a blue arrow. Likelihood values for branches with a value less than 0.9 are shown.