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. 2018 Jul 12;8:10552. doi: 10.1038/s41598-018-28908-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Output of assessment of strength and direction of selection on chromosomal genes. (A) A plot of pairwise dN/dS versus divergence time (dS) over all chromosomal genes. (B) A plot of dN versus dS for all chromosomal genes. Those genes encoding surface exposed proteins, or other genes which are putatively immunogenic (see Table S2) are shown in blue, all other genes are shown in red. The lines represent two interpretations of the neutral expectation (dN/dS = 1 and 0.1). The single gene with dN/dS > 1 is rpsH which encodes a ribosomal protein hence would be expected to be under strong stabilising selection.