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. 2018 Jul 12;8:10549. doi: 10.1038/s41598-018-28910-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Shock wave exposure decreases lifespan. (a) Lifespans of N2 C. elegans raised in liquid cultures following shock wave exposure. (b) Lifespans of N2 worms raised on NGM agar plates following shock wave exposure. (c) Lifespans of N2 worms exposed to shock waves in S-medium or polyvinyl alcohol. (d) Lifespans of N2 worms exposed to shock waves in S-medium or polyvinyl alcohol, followed by a short washing step. (e) Lifespans of daf-16(m26) worms following shock wave exposure. (f) Lifespans of N2 worms at 11 °C following shock wave exposure. (g) Lifespans of daf-2(e1370) worms at 11 °C following exposure to shock waves. In all panels, vertical dotted lines represent 50% lifespan of control. Arrows and horizontal dotted lines represent the percent survival of each test group at time of the corresponding 50% control survival. N > 500 worms in all experiment groups (the exact numbers are provided in the Methods section).