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. 2018 Jul 12;8:10562. doi: 10.1038/s41598-018-28763-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

In response to the activation of their CB₁-Rs, cultured spinal astrocytes produce and release 2-AG, but not anandamide, oleoylethanolamide (OEA) or palmitoylethanolamide (PEA). Box-plots showing the quantities of 2-AG (a), anandamide (b), OEA (c) and PEA (d) in pmol/mg measured with LC-APCI-MS in cultured wild-type mouse spinal astrocytes. Quantities of endocannabinoids were measured in control conditions and following the application of 10 µM WIN or the Ca²⁺ ionophore A23187 (5 µM). The effects of 5 µM AM251 and 5 µM BAPTA-1-AM pretreatment on WIN-evoked 2-AG production are also illustrated. Asterisks indicate significant differences between the quantities of 2-AG measured under different experimental conditions (p = 0.023, ctrl vs WIN; p = 0.040, WIN vs AM + WIN; p = 0.041, WIN vs BAPTA + WIN).