Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 12;8:10503. doi: 10.1038/s41598-018-28963-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Spontaneous emergence of cellular senescence in populations of MDA-MB-231 breast cancer cells. (a) Schematic diagram illustrating a small polydimethylsiloxane (PDMS) well in a culture dish. Detached cells (red) are put into the well; the PDMS is removed after 4 5 hours of planting. (b) Phase-contrast snapshot images taken at two different times after the initial seeding; ‘holes’ are the areas occupied by senescent cells. (c) Close-up views of an exemplary cellular senescence (outlined by dashed lines) developing in time. (d) The growing (followed by apoptosis) areal sizes of several different senescent cells. (e) An exemplary confocal 3D immuno-stained image of a senescent cell showing the distribution of filamentous actin marker phalloidin (green) and nuclei marker Hoechst staining (blue). Each side view shows a slice cut in z-direction at the highlighted locations (stretched three times for a better visualization). The 3D image consists of a confocal z-stack of 49 images. [scale bars: (b) 500 μm; (c) and (e) 100 μm]