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. 2018 Jul 12;8:10577. doi: 10.1038/s41598-018-28911-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Global DNA/RNA (hydroxy)methylation modulation in rat liver upon exposure to PAH mixture. DNA methylation (m⁵C-DNA) (A) and hydroxymethylation (hm⁵C-DNA) (C) levels, RNA methylation (m⁵C-RNA) (B) and hydroxymethylation (hm⁵C-RNA) (D) levels variation over different doses (n = 8, excepting for dose 40 µg/kg b.w. were n = 7) are depicted. The means together with the standard error of the mean are shown as grey columns and bars, respectively. Based on the ANOVA statistical analysis, p- values (*<0.05; ** < 0.01; ***<0.001) have been calculated using a Bonferroni’s multiple comparisons test, as detailed in the section 2.3, in order to assess the statistical significance of the observed mean values as compared to the control group.