Figure 5.

The effect of iron chelators and a reducing agent can be eliminated by addition of excess iron. Hda deficient cells were spread on minimal rich medium agar plates containing iron (III) chloride at a final concentration of 3 μM, iron (II) perchlorate at a final concentration of 3 μM or iron (II) perchlorate at a final concentration of 200 μM and tested against metal chelators and antioxidant. 5 μl of 10 mM DFO, 10 mM phenanthroline, 10 mM EDTA, 300 mM bipyridyl or 650 mM DTT was dispensed in separated wells.