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. 2018 Jul 12;8:10567. doi: 10.1038/s41598-018-28926-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Axial (left column) and Coronal (right column) high resolution T_2w MR images of a representative healthy control C57BL/6 mouse (first row) and representative TRAMP mice at three development stages namely: early stage (second row), middle stage (third row), differentiated late stage (fourth row) (Thin white arrow indicates prostate, thin yellow arrow indicates tumoral lesion in seminal vesicles, i.e. phyllodes tumor). (fifth row) Poorly differentiated late stage, PD (thick white arrow = poorly differentiated PCa region, red arrow = differentiated PCa, green arrow = invaded seminal vesicles); (B) Histograms reporting prostate’s size for the investigated groups. Data are reported as mean ± SD; (C) Representative H/E histological images of prostate tissue in healthy and TRAMP mice and of healthy seminal vesicles and phyllodes tumour (Magnification 200X. Poorly differentiated tumor image on the left: magnification 100X. Poorly differentiated tumor image on the right: magnification 25X). White outlines indicate areas of necrosis. PD = poorly differentiated. (n = 8).