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. 2018 Jul 12;8:10558. doi: 10.1038/s41598-018-28970-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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COX-2 in cortical microvasculature during abstinence from Meth. (A) 3 days of abstinence from Meth increased COX-2 in CUS + Meth compared to CUS. (*p < 0.05) and Meth (^#p < 0.01) n = 6–8/group. Middle panel shows representative blot probed for COX-2 after 3 days of abstinence from Meth and the bottom panel shows the loading control α-tubulin. (B) 7 days of abstinence from Meth increased COX-2 in CUS + Meth compared to CUS (*p < 0.05) n = 6–8/group. Middle panel shows representative blot probed for COX-2 after 7 days of abstinence from Meth and the bottom panel shows the blot for loading control α-tubulin. COX-2 and α-tubulin band images presented have been vertically sliced in order to juxtapose lanes that were non-adjacent in the blot. Full-length blots are presented in Supplementary Figures S2A and S2B.