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. 2018 Jul 12;8:10492. doi: 10.1038/s41598-018-28630-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SNP sharing among the seven strains of T. pseudonana. Cladogram based on a total of 468,117 refined SNPs, accounting for 82% of SNP positions, identified across all 7 strains (Supp. 9). The tree is rooted at the common ancestor of all seven isolates, with each split selected to maximize the number of SNPs shared by isolates within each subtree and minimize sharing across subtrees. Internal branch lengths are the numbers of SNPs shared by all isolates in the subtree below that branch. Terminal branch lengths are the numbers of private SNPs found in each isolate. The red arrow reflects an inferred evolutionary event in which inbreeding resulted in a significant loss of heterozygosity (LoH) and concomitant loss of sexual reproduction (LoS) that founded the L-clade. Scale: number of SNPs.