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. 2018 Jul 12;8:10548. doi: 10.1038/s41598-018-28439-1

Figure 6.

Figure 6

Effects of probucol on the modulation of Smad1 phosphorylation and ECM proteins expression in a diabetic condition. Thirty μM probucol or DMSO were added to MCs which were pre-exposed to BSA or AGEs for 36 h, and incubated for additional 12 h at 37 °C. (a) Equal amounts of cell lysates were subjected to western blot. GAPDH was used as a loading control. Data are representative of at least three independent experiments. (b) Optical densitometry of these proteins in western blot was shown. The values are expressed in mean ± S.E. (NS, not significant, *p < 0.05, t test). ctl and pr stand for DMSO- and probucol-treated MCs, respectively. Uncropped scans are presented in Supplementary Fig. S8.