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. 2018 Jul 12;8:10579. doi: 10.1038/s41598-018-28605-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Suppressed expression of osteoblast differentiation markers in primary mouse osteoblasts by introduction of Mct1 siRNA. Control or Mct1 siRNA was introduced into primary osteoblasts isolated from mouse calvaria. Osteoblasts were cultured for 72 (a,b) or 48 (c) hours in the absence (−) or presence (+) of BMP-2 (300 ng/mL). (a) ALP activity, detected by staining. (b) ALP activity in cell lysates. (c) Expression of mRNAs for Tnap, Bglap, Runx2, and Sp7 were analyzed by real-time RT-PCR, then normalized against that of Gapdh, with the results indicated as relative values. (b,c) Data are expressed as the mean ± SD (n = 3–5). *, **Significantly different from control group (*p < 0.05, **p < 0.01).