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. 2018 Jul 12;8:10579. doi: 10.1038/s41598-018-28605-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Osteoblastic differentiation suppressed by Mct1 siRNA recovered by introduction of Trp53 siRNA. C2C12 cells were introduced with control or Mct1 siRNA in combination with Trp53 siRNA or Klf4 siRNA, then cultured for 72 (a) or 48 (b) hours in the absence (−) or presence (+) of BMP-2 (300 ng/mL). (a) ALP activity in the cultures was detected by staining. (b) ALP activity in lysates of C2C12 cells. (c) Expression of mRNAs for Tnap and Sp7 were analyzed by real-time RT-PCR, then normalized against that of Gapdh, with the results indicated as relative values. (b,c) Data are expressed as the mean ± SD (n = 3–5). *Significant difference between groups (*p < 0.05).