Figure 4.

Lentiviral vector-gra15_II delivery ameliorated hepatic granulomas and fibrosis in schistosomiasis mice. Mice were killed under euthanasia at 8 weeks post-infection, and then the blood and the liver were collected. The appearances of liver were exhibited (A). Liver tissue section was stained with hematoxylin and eosin and the granulomas area was showed with quantitative analysis (B,C). Liver tissue section was stained with Masson and the fibrosis area was showed with quantitative analysis (D,E). Col Ι protein level in liver was evaluated by IHC with quantitative analysis of optical density (F,G) and Col Ι gene expression in liver was analyzed by quantitative real-time PCR (H). The HYP content in liver was checked by hydroxyproline assay kit (I). Sera hyaluronic acid level was assayed by enzyme-linked immunosorbent assay (J). Eggs in liver tissue were counted under light microscope (K). Data represent mean ± SD from multi-group experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 (n = 4 each group).