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. 2018 Jul 6;9:1572. doi: 10.3389/fimmu.2018.01572

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Copyright © 2018 Liu, Jin, Tao, Yu, Du, Wang, Luo, Xing, Xu, Shen and Chu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lentiviral vector-gra15_II-M delivery decreased hepatic stellate cell (HSC) activation by the reduced expression of transforming growth factor-beta1 (TGF-β1). TGF-β1 protein level in liver was evaluated by IHC with quantitative analysis of optical density (A,C); the protein level of α-SMA in liver was evaluated by IHC with quantitative analysis of optical density (B,E). Liver RNA was extracted and the expression of TGF-β1 and α-SMA were analyzed by quantitative real-time PCR (D,F). Data represent mean ± SD from multi-group experiments. **p < 0.01 and ***p < 0.001 (n = 4 each group).