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. 2018 Jul 6;9:1557. doi: 10.3389/fimmu.2018.01557

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Copyright © 2018 Zhang, Feng, Fu, Liu, Yu, Sun, She, Li, Zhao, Liu, Liu, Liu, Liu, Li and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-338-3p targeted factor X (FX) to suppress macrophage migration. (A) Real-time PCR detected that the mRNA expression of FX decreased after transfection with miR-338-3p mimics in G1124 cells (mean ± SEM, *p < 0.05). (B) Western blotting detected reduced FX expression in cell lysates and supernatants after G1124 cells were transfected with miR-338-3p mimics. (C) The luciferase reporter assay showed that miR-338-3p decreased luciferase activity (mean ± SEM, **p < 0.01). (D) The number of phorbol 12-myristate 13-acetate (PMA)-primed THP-1 cells migrating toward G1124 cell supernatants decreased when G1124 cells were transfected with miR-338-3p mimics (mean ± SEM, *p < 0.01). (E) Real-time PCR detected the expression of M1 and M2 markers in THP-1 cells after incubation with G1124 cell supernatants transfected with NC or miR-338-3p mimics (mean ± SEM). (F,G) Flow cytometry analysis showed that the proportion of CD11b⁺CD80⁺ M1 macrophages and CD11b⁺CD206⁺ M2 macrophages did not changed in THP-1 cells treated with supernatants from G1124 cells transfected with miR-338-3p compared with NC. (G) Bar graph of CD11b⁺CD80⁺ and CD11b⁺CD206⁺ cell proportion in panel (F).