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. 2018 Jul 6;9:1528. doi: 10.3389/fmicb.2018.01528

FIGURE 2.

FIGURE 2

Deletion of the Mrmep1 and Mrmep2 gene in M. robertsii. (A–D) PCR analysis of the deleted genes using the primers Mrmep1-F and Mrmep1-R (A), the primers Mrmep2-F and Mrmep2-R (B), the primers bar-F and bar-R (C), and the primers ben-F and ben-R (D). M, Marker; 1, WT; 2, ΔMrmep1; 3, ΔMrmep2; 4, ΔMrmep1 ΔMrmep2; 5, cpΔMrmep1; 6, cpΔMrmep2. (E,F) PCR analysis for the bar (E) or ben (F) genes. M, Marker; 1, WT; 2, ΔMrmep1; 3, ΔMrmep2; 4, ΔMrmep1ΔMrmep2. (G) RT-PCR analysis for the deleted genes. M, Marker; 1 and 4, WT; 2, ΔMrmep1; 3 and 6, ΔMrmep1ΔMrmep2; 5, ΔMrmep2. (H,I) Southern blotting hybridization with bar or ben gene probes. 1, WT; 2, ΔMrmep1; 3, ΔMrmep2; 4, ΔMrmep1ΔMrmep2; 5, cpΔMrmep1; 6, cpΔMrmep2. Further information on primers can be found in Table 1.